Journal: Materials Today Bio

Article Title: MicroRNA-493-5p engineered exosomes delivered via piezoelectric microneedles for epigenetic modulation of macrophages in diabetic wound healing

doi: 10.1016/j.mtbio.2026.102931

Figure Lengend Snippet: Preparation and characterization of Ag/GOx@GelMA/ZnO MN @EXO@miR . (A) Fabrication procedure of Ag/GOx@GelMA/ZnO MN @EXO@miR . (B,C) The appearance of GelMA MN and Ag/GOx@GelMA/ZnO MN patch under a low-magnification field of view. (D)Scanning electron microscopy (SEM) images to verify the needle shape of Ag/GOx@GelMA/ZnO MN; Scale bar: 1000 μm, 500 μm, 300 μm. (E) Confocal microscopy image of GelMA MN (red) loading EXO (green). (F) Force-displacement curve from compression of GelMA MN and Ag/GOx@GelMA/ZnO MN. (G) SEM images to verify the internal structure of Ag/GOx@GelMA/ZnO MN; Scale bar: 150 μm, 50 μm. (H) EDS to verify the element composition of Ag/GOx@GelMA/ZnO MN; Scale bar: 50 μm. (I) Degradation curve of GelMA MN and Ag/GOx@GelMA/ZnO MN determined by dry weight. (J) EXO release curve of GelMA MN and Ag/GOx@GelMA/ZnO MN analyzed using the bicinchoninic acid (BCA) assay. (K) Piezoelectric effect test of Ag/GOx@GelMA/ZnO MN. (L) Physical and electron microscopy images of MRSA and E. coli cultured for 12 h; Scale bar: 1 cm, 500 nm. (M) DFCH-DA staining of HUVECs treated with liquid GelMA MN or Ag/GOx@GelMA/ZnO MN extracts. (N) Live and dead cell assays of HUVECs treated with liquid GelMA MN or Ag/GOx@GelMA/ZnO MN extracts.

Article Snippet: Mechanical strength was tested via compression assay (Instron 5944, 0.5 mm/min).

Techniques: Electron Microscopy, Confocal Microscopy, BIA-KA, Cell Culture, Staining

Journal: Materials Today Bio

Article Title: A sequential and synergistic release sacrificial microgels scaffold for endometrial niche reprogramming and fertility restoration

doi: 10.1016/j.mtbio.2026.103235

Figure Lengend Snippet: Biocompatibility and Physicochemical Properties of the MSH Scaffold. (A) Hemolysis test results. One-way ANOVA analysis was utilized for the statistical Analysis, n = 3, ∗∗∗p < 0.001 compared with CON group. (B) Fluorescence images of HUVECs co-cultured with the MSH scaffold for 1, 2, and 3 days by Calcein AM staining. Scale bar, 100 μm. (C) Amplitude sweep curves of GelMA and MSH materials. (D) Alternate step strain curves of GelMA and MSH materials. (E) Shear-thinning profile of the GelMA and MSH precursor solution. (F) compression curves of MSH scaffold under 25 °Cand under 37 °C. (G, H) Swelling and loss curves of GelMA and MSH materials. (I) Protein release curve of the MSH materials. n = 3. (J, K) Release kinetic curves of VEGF and EGF in the MSH materials. n = 3. GelMA: GelMA hydrogel; MSH: Gelatin microgels hydrogel; PRP: Pure PRP; PMSH: PRP gelatin microgels hydrogel.

Article Snippet: Compression tests and visco-elastic properties : The compressive deformation potential of the bracket was measured on a universal compression tester (5944, Instron, USA), with a strain rate set at 5 mm per minute.

Techniques: Fluorescence, Cell Culture, Staining, Shear

Journal: Orthopaedic Journal of Sports Medicine

Article Title: Pediatric Meniscotibial Ligament Complex Anatomy and Biomechanics

doi: 10.1177/23259671251367060

Figure Lengend Snippet: Defining the meniscotibial ligament complex (MTLC) and isolating its anterior, central, and posterior aspects for biomechanical testing. (A) Schematic of a coronal cross section of half of a knee. The MTLC is depicted in red. The meniscocapsular recess space is indicated between the MTLC and the meniscocapsular complex (MCC, or joint capsule). The meniscocapsular recess space measurements were taken as indicated by the yellow line from the inferiormost point of the recess to the top of the meniscus, measured in the axial direction. (B) Gross examination of pediatric knee specimen indicating the distinct space that separates the MTLC and the MCC in the posterior aspect of the medial and lateral menisci. The distinct space also exists in the anterior and central regions but is only indicated in the posterior aspect in this image. The large white arrowhead indicates the popliteal hiatus. The small red lines indicate the MTLC, the small blue arrows indicate the MCC, and the small red circles show the meniscocapsular recess. MM, medial meniscus; LM, lateral meniscus; Po, popliteal tendon. (C) Axial schematic of radial meniscal transections, adapted from Dingel et al. Areas with red dashed line indicate where a scalpel was used to release the meniscus roots or transect the meniscus/MTLC complex into thirds. Numbered regions correspond to the segments of the meniscus/MTLC complex that subsequently underwent biomechanical testing: 1. Medial posterior; 2. Medial central; 3. Medial anterior; 4. Lateral anterior; 5. Lateral central; 6. Lateral posterior. ACL, anterior cruciate ligament; AL, anterolateral; AM, anteromedial. (D) Clamp on the anterior one-third of the lateral meniscus pulling vertically on the MTLC to failure. The tibia was potted in fiberglass resin and was vertically mounted on the Instron 5944 test frame.

Article Snippet: Once dried, each tibial specimen was mounted on an Instron 5944 electromechanical load frame with a 2-kN load cell.

Techniques: